quality control (qc) samples Search Results


quality control sera  (ZeptoMetrix corporation)
93
ZeptoMetrix corporation quality control sera
Quality Control Sera, supplied by ZeptoMetrix corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/quality control sera/product/ZeptoMetrix corporation
Average 93 stars, based on 1 article reviews
quality control sera - by Bioz Stars, 2026-03
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5 hydroxymethylfurfural hmf  (Chem Impex International)
96
Chem Impex International 5 hydroxymethylfurfural hmf
5 Hydroxymethylfurfural Hmf, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/5 hydroxymethylfurfural hmf/product/Chem Impex International
Average 96 stars, based on 1 article reviews
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levey- jennings chart westgard rules  (WesTgard QC)
90
WesTgard QC levey- jennings chart westgard rules
Levey Jennings Chart Westgard Rules, supplied by WesTgard QC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/levey- jennings chart westgard rules/product/WesTgard QC
Average 90 stars, based on 1 article reviews
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quality control plasma samples  (RECIPE Chemicals and Instruments)
90
RECIPE Chemicals and Instruments quality control plasma samples
Quality Control Plasma Samples, supplied by RECIPE Chemicals and Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/quality control plasma samples/product/RECIPE Chemicals and Instruments
Average 90 stars, based on 1 article reviews
quality control plasma samples - by Bioz Stars, 2026-03
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metabolomics quality control (qc) kit  (Cambridge Isotope Laboratories)
90
Cambridge Isotope Laboratories metabolomics quality control (qc) kit
Energetics of Eμ- Myc lymphoma cells during disease progression. A, Time schematic of when Eμ- Myc lymphoma-bearing C57BL/6J mice were analyzed following transplant; control (C, day 0), early (E, day 7), and late (L, day 14). B, Total splenocyte number ( n = 5–7 mice) from Eμ- Myc lymphoma-bearing mice. C, Tumor cell size and ( D ) mitochondrial biomass, measured by flow cytometry, of Eμ- Myc lymphoma cells versus normal splenic B cells ( n = 5–9 mice). E, PCA and ( F ) heat map of differentially abundant metabolites of Eμ- Myc lymphoma cells derived from spleens, at the indicated time points following lymphoma transplant, versus normal splenic B cells using untargeted <t>metabolomics</t> analysis ( n = 4 mice/cohort). G, Levels of select metabolites in the glutaminolysis and glycolysis pathways, and in the TCA cycle, from the metabolomics data. αKG, alpha-ketoglutarate; G-6-P, glucose-6-phosphate; F-6-P, fructose-6-phosphate; DHAP, dihydroxyacetone phosphate; G-3-P, glyceraldehyde-3-phosphate; 2-PGA, 2-phosphoglyceric acid. Experiments in B to D were analyzed by one-way ANOVA and Dunnett multiple comparisons and were two independent experiments combined. Metabolomics data in E to G were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.001; n.s., not significant; for G , *, FDR < 0.05).
Metabolomics Quality Control (Qc) Kit, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/metabolomics quality control (qc) kit/product/Cambridge Isotope Laboratories
Average 90 stars, based on 1 article reviews
metabolomics quality control (qc) kit - by Bioz Stars, 2026-03
90/100 stars
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lyophilized normal abnormal quality control (qc) samples  (Siemens AG)
90
Siemens AG lyophilized normal abnormal quality control (qc) samples
Energetics of Eμ- Myc lymphoma cells during disease progression. A, Time schematic of when Eμ- Myc lymphoma-bearing C57BL/6J mice were analyzed following transplant; control (C, day 0), early (E, day 7), and late (L, day 14). B, Total splenocyte number ( n = 5–7 mice) from Eμ- Myc lymphoma-bearing mice. C, Tumor cell size and ( D ) mitochondrial biomass, measured by flow cytometry, of Eμ- Myc lymphoma cells versus normal splenic B cells ( n = 5–9 mice). E, PCA and ( F ) heat map of differentially abundant metabolites of Eμ- Myc lymphoma cells derived from spleens, at the indicated time points following lymphoma transplant, versus normal splenic B cells using untargeted <t>metabolomics</t> analysis ( n = 4 mice/cohort). G, Levels of select metabolites in the glutaminolysis and glycolysis pathways, and in the TCA cycle, from the metabolomics data. αKG, alpha-ketoglutarate; G-6-P, glucose-6-phosphate; F-6-P, fructose-6-phosphate; DHAP, dihydroxyacetone phosphate; G-3-P, glyceraldehyde-3-phosphate; 2-PGA, 2-phosphoglyceric acid. Experiments in B to D were analyzed by one-way ANOVA and Dunnett multiple comparisons and were two independent experiments combined. Metabolomics data in E to G were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.001; n.s., not significant; for G , *, FDR < 0.05).
Lyophilized Normal Abnormal Quality Control (Qc) Samples, supplied by Siemens AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lyophilized normal abnormal quality control (qc) samples/product/Siemens AG
Average 90 stars, based on 1 article reviews
lyophilized normal abnormal quality control (qc) samples - by Bioz Stars, 2026-03
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oligo-probes based on 18 different phage lambda sequences and 63 different quality controls (qc)  (CombiMatrix)
90
CombiMatrix oligo-probes based on 18 different phage lambda sequences and 63 different quality controls (qc)
Energetics of Eμ- Myc lymphoma cells during disease progression. A, Time schematic of when Eμ- Myc lymphoma-bearing C57BL/6J mice were analyzed following transplant; control (C, day 0), early (E, day 7), and late (L, day 14). B, Total splenocyte number ( n = 5–7 mice) from Eμ- Myc lymphoma-bearing mice. C, Tumor cell size and ( D ) mitochondrial biomass, measured by flow cytometry, of Eμ- Myc lymphoma cells versus normal splenic B cells ( n = 5–9 mice). E, PCA and ( F ) heat map of differentially abundant metabolites of Eμ- Myc lymphoma cells derived from spleens, at the indicated time points following lymphoma transplant, versus normal splenic B cells using untargeted <t>metabolomics</t> analysis ( n = 4 mice/cohort). G, Levels of select metabolites in the glutaminolysis and glycolysis pathways, and in the TCA cycle, from the metabolomics data. αKG, alpha-ketoglutarate; G-6-P, glucose-6-phosphate; F-6-P, fructose-6-phosphate; DHAP, dihydroxyacetone phosphate; G-3-P, glyceraldehyde-3-phosphate; 2-PGA, 2-phosphoglyceric acid. Experiments in B to D were analyzed by one-way ANOVA and Dunnett multiple comparisons and were two independent experiments combined. Metabolomics data in E to G were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.001; n.s., not significant; for G , *, FDR < 0.05).
Oligo Probes Based On 18 Different Phage Lambda Sequences And 63 Different Quality Controls (Qc), supplied by CombiMatrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oligo-probes based on 18 different phage lambda sequences and 63 different quality controls (qc)/product/CombiMatrix
Average 90 stars, based on 1 article reviews
oligo-probes based on 18 different phage lambda sequences and 63 different quality controls (qc) - by Bioz Stars, 2026-03
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dna quality control (qc) particles  (Becton Dickinson)
90
Becton Dickinson dna quality control (qc) particles
Energetics of Eμ- Myc lymphoma cells during disease progression. A, Time schematic of when Eμ- Myc lymphoma-bearing C57BL/6J mice were analyzed following transplant; control (C, day 0), early (E, day 7), and late (L, day 14). B, Total splenocyte number ( n = 5–7 mice) from Eμ- Myc lymphoma-bearing mice. C, Tumor cell size and ( D ) mitochondrial biomass, measured by flow cytometry, of Eμ- Myc lymphoma cells versus normal splenic B cells ( n = 5–9 mice). E, PCA and ( F ) heat map of differentially abundant metabolites of Eμ- Myc lymphoma cells derived from spleens, at the indicated time points following lymphoma transplant, versus normal splenic B cells using untargeted <t>metabolomics</t> analysis ( n = 4 mice/cohort). G, Levels of select metabolites in the glutaminolysis and glycolysis pathways, and in the TCA cycle, from the metabolomics data. αKG, alpha-ketoglutarate; G-6-P, glucose-6-phosphate; F-6-P, fructose-6-phosphate; DHAP, dihydroxyacetone phosphate; G-3-P, glyceraldehyde-3-phosphate; 2-PGA, 2-phosphoglyceric acid. Experiments in B to D were analyzed by one-way ANOVA and Dunnett multiple comparisons and were two independent experiments combined. Metabolomics data in E to G were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.001; n.s., not significant; for G , *, FDR < 0.05).
Dna Quality Control (Qc) Particles, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna quality control (qc) particles/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
dna quality control (qc) particles - by Bioz Stars, 2026-03
90/100 stars
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long-term reference quality control samples  (Metabolon Inc)
90
Metabolon Inc long-term reference quality control samples
Energetics of Eμ- Myc lymphoma cells during disease progression. A, Time schematic of when Eμ- Myc lymphoma-bearing C57BL/6J mice were analyzed following transplant; control (C, day 0), early (E, day 7), and late (L, day 14). B, Total splenocyte number ( n = 5–7 mice) from Eμ- Myc lymphoma-bearing mice. C, Tumor cell size and ( D ) mitochondrial biomass, measured by flow cytometry, of Eμ- Myc lymphoma cells versus normal splenic B cells ( n = 5–9 mice). E, PCA and ( F ) heat map of differentially abundant metabolites of Eμ- Myc lymphoma cells derived from spleens, at the indicated time points following lymphoma transplant, versus normal splenic B cells using untargeted <t>metabolomics</t> analysis ( n = 4 mice/cohort). G, Levels of select metabolites in the glutaminolysis and glycolysis pathways, and in the TCA cycle, from the metabolomics data. αKG, alpha-ketoglutarate; G-6-P, glucose-6-phosphate; F-6-P, fructose-6-phosphate; DHAP, dihydroxyacetone phosphate; G-3-P, glyceraldehyde-3-phosphate; 2-PGA, 2-phosphoglyceric acid. Experiments in B to D were analyzed by one-way ANOVA and Dunnett multiple comparisons and were two independent experiments combined. Metabolomics data in E to G were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.001; n.s., not significant; for G , *, FDR < 0.05).
Long Term Reference Quality Control Samples, supplied by Metabolon Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/long-term reference quality control samples/product/Metabolon Inc
Average 90 stars, based on 1 article reviews
long-term reference quality control samples - by Bioz Stars, 2026-03
90/100 stars
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standard quality control (qc) sample  (National Institute of Standards and Technology)
90
National Institute of Standards and Technology standard quality control (qc) sample
Energetics of Eμ- Myc lymphoma cells during disease progression. A, Time schematic of when Eμ- Myc lymphoma-bearing C57BL/6J mice were analyzed following transplant; control (C, day 0), early (E, day 7), and late (L, day 14). B, Total splenocyte number ( n = 5–7 mice) from Eμ- Myc lymphoma-bearing mice. C, Tumor cell size and ( D ) mitochondrial biomass, measured by flow cytometry, of Eμ- Myc lymphoma cells versus normal splenic B cells ( n = 5–9 mice). E, PCA and ( F ) heat map of differentially abundant metabolites of Eμ- Myc lymphoma cells derived from spleens, at the indicated time points following lymphoma transplant, versus normal splenic B cells using untargeted <t>metabolomics</t> analysis ( n = 4 mice/cohort). G, Levels of select metabolites in the glutaminolysis and glycolysis pathways, and in the TCA cycle, from the metabolomics data. αKG, alpha-ketoglutarate; G-6-P, glucose-6-phosphate; F-6-P, fructose-6-phosphate; DHAP, dihydroxyacetone phosphate; G-3-P, glyceraldehyde-3-phosphate; 2-PGA, 2-phosphoglyceric acid. Experiments in B to D were analyzed by one-way ANOVA and Dunnett multiple comparisons and were two independent experiments combined. Metabolomics data in E to G were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.001; n.s., not significant; for G , *, FDR < 0.05).
Standard Quality Control (Qc) Sample, supplied by National Institute of Standards and Technology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/standard quality control (qc) sample/product/National Institute of Standards and Technology
Average 90 stars, based on 1 article reviews
standard quality control (qc) sample - by Bioz Stars, 2026-03
90/100 stars
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calibrators and quality control samples  (Scipac Ltd)
90
Scipac Ltd calibrators and quality control samples
Energetics of Eμ- Myc lymphoma cells during disease progression. A, Time schematic of when Eμ- Myc lymphoma-bearing C57BL/6J mice were analyzed following transplant; control (C, day 0), early (E, day 7), and late (L, day 14). B, Total splenocyte number ( n = 5–7 mice) from Eμ- Myc lymphoma-bearing mice. C, Tumor cell size and ( D ) mitochondrial biomass, measured by flow cytometry, of Eμ- Myc lymphoma cells versus normal splenic B cells ( n = 5–9 mice). E, PCA and ( F ) heat map of differentially abundant metabolites of Eμ- Myc lymphoma cells derived from spleens, at the indicated time points following lymphoma transplant, versus normal splenic B cells using untargeted <t>metabolomics</t> analysis ( n = 4 mice/cohort). G, Levels of select metabolites in the glutaminolysis and glycolysis pathways, and in the TCA cycle, from the metabolomics data. αKG, alpha-ketoglutarate; G-6-P, glucose-6-phosphate; F-6-P, fructose-6-phosphate; DHAP, dihydroxyacetone phosphate; G-3-P, glyceraldehyde-3-phosphate; 2-PGA, 2-phosphoglyceric acid. Experiments in B to D were analyzed by one-way ANOVA and Dunnett multiple comparisons and were two independent experiments combined. Metabolomics data in E to G were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.001; n.s., not significant; for G , *, FDR < 0.05).
Calibrators And Quality Control Samples, supplied by Scipac Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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multirule quality control method  (WesTgard QC)
90
WesTgard QC multirule quality control method
Energetics of Eμ- Myc lymphoma cells during disease progression. A, Time schematic of when Eμ- Myc lymphoma-bearing C57BL/6J mice were analyzed following transplant; control (C, day 0), early (E, day 7), and late (L, day 14). B, Total splenocyte number ( n = 5–7 mice) from Eμ- Myc lymphoma-bearing mice. C, Tumor cell size and ( D ) mitochondrial biomass, measured by flow cytometry, of Eμ- Myc lymphoma cells versus normal splenic B cells ( n = 5–9 mice). E, PCA and ( F ) heat map of differentially abundant metabolites of Eμ- Myc lymphoma cells derived from spleens, at the indicated time points following lymphoma transplant, versus normal splenic B cells using untargeted <t>metabolomics</t> analysis ( n = 4 mice/cohort). G, Levels of select metabolites in the glutaminolysis and glycolysis pathways, and in the TCA cycle, from the metabolomics data. αKG, alpha-ketoglutarate; G-6-P, glucose-6-phosphate; F-6-P, fructose-6-phosphate; DHAP, dihydroxyacetone phosphate; G-3-P, glyceraldehyde-3-phosphate; 2-PGA, 2-phosphoglyceric acid. Experiments in B to D were analyzed by one-way ANOVA and Dunnett multiple comparisons and were two independent experiments combined. Metabolomics data in E to G were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.001; n.s., not significant; for G , *, FDR < 0.05).
Multirule Quality Control Method, supplied by WesTgard QC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Image Search Results


Energetics of Eμ- Myc lymphoma cells during disease progression. A, Time schematic of when Eμ- Myc lymphoma-bearing C57BL/6J mice were analyzed following transplant; control (C, day 0), early (E, day 7), and late (L, day 14). B, Total splenocyte number ( n = 5–7 mice) from Eμ- Myc lymphoma-bearing mice. C, Tumor cell size and ( D ) mitochondrial biomass, measured by flow cytometry, of Eμ- Myc lymphoma cells versus normal splenic B cells ( n = 5–9 mice). E, PCA and ( F ) heat map of differentially abundant metabolites of Eμ- Myc lymphoma cells derived from spleens, at the indicated time points following lymphoma transplant, versus normal splenic B cells using untargeted metabolomics analysis ( n = 4 mice/cohort). G, Levels of select metabolites in the glutaminolysis and glycolysis pathways, and in the TCA cycle, from the metabolomics data. αKG, alpha-ketoglutarate; G-6-P, glucose-6-phosphate; F-6-P, fructose-6-phosphate; DHAP, dihydroxyacetone phosphate; G-3-P, glyceraldehyde-3-phosphate; 2-PGA, 2-phosphoglyceric acid. Experiments in B to D were analyzed by one-way ANOVA and Dunnett multiple comparisons and were two independent experiments combined. Metabolomics data in E to G were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.001; n.s., not significant; for G , *, FDR < 0.05).

Journal: Cancer Immunology Research

Article Title: TCR-Independent Metabolic Reprogramming Precedes Lymphoma-Driven Changes in T-cell Fate

doi: 10.1158/2326-6066.CIR-21-0813

Figure Lengend Snippet: Energetics of Eμ- Myc lymphoma cells during disease progression. A, Time schematic of when Eμ- Myc lymphoma-bearing C57BL/6J mice were analyzed following transplant; control (C, day 0), early (E, day 7), and late (L, day 14). B, Total splenocyte number ( n = 5–7 mice) from Eμ- Myc lymphoma-bearing mice. C, Tumor cell size and ( D ) mitochondrial biomass, measured by flow cytometry, of Eμ- Myc lymphoma cells versus normal splenic B cells ( n = 5–9 mice). E, PCA and ( F ) heat map of differentially abundant metabolites of Eμ- Myc lymphoma cells derived from spleens, at the indicated time points following lymphoma transplant, versus normal splenic B cells using untargeted metabolomics analysis ( n = 4 mice/cohort). G, Levels of select metabolites in the glutaminolysis and glycolysis pathways, and in the TCA cycle, from the metabolomics data. αKG, alpha-ketoglutarate; G-6-P, glucose-6-phosphate; F-6-P, fructose-6-phosphate; DHAP, dihydroxyacetone phosphate; G-3-P, glyceraldehyde-3-phosphate; 2-PGA, 2-phosphoglyceric acid. Experiments in B to D were analyzed by one-way ANOVA and Dunnett multiple comparisons and were two independent experiments combined. Metabolomics data in E to G were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.001; n.s., not significant; for G , *, FDR < 0.05).

Article Snippet: The Metabolomics Quality Control (QC) Kit, which contains 14 stable isotope-labeled metabolite standards (Cambridge Isotope Labs), included the following compounds: L-alanine ( 13 C3, 99% purity), L-leucine ( 13 C6, 99%), L-phenylalanine ( 13 C6, 99%), L-tryptophan ( 13 C11, 99%), L-tyrosine ( 13 C6, 99%), caffeine ( 13 C3, 99%), D-glucose ( 13 C6, 99%), benzoate ( 13 C6, 99%), citrate ( 13 C3, 99%), octanoate ( 13 C8, 99%), propionate ( 13 C3, 99%), stearic acid ( 13 C18, 98%), succinic acid ( 13 C4, 99%), and D-sucrose ( 13 C6, 98%).

Techniques: Biomarker Discovery, Control, Flow Cytometry, Derivative Assay

Metabolic reprogramming of CD4 + T cells occurs early during Eμ- Myc lymphoma progression. A, Glu-Cy5 uptake capacity in CD4 + T cells from Eμ- Myc lymphoma-bearing C57BL/6J mice at the indicated timepoints, labeled ex vivo ( n = 5–10 mice). C, Control; E, early; L, late. B, Glu-Cy5 uptake capacity in CD45.2 + CD4 + T cells transferred into normal or Eμ- Myc lymphoma-bearing CD45.1 + congenic mice as in ( n = 5 mice). C, PCA based on untargeted metabolomics of isolated CD4 + T cells from Eμ- Myc lymphoma-bearing C57BL/6J mice at the indicated timepoints ( n = 6–7 mice). D, Heatmap of 59 metabolites that were differentially abundant by ANOVA at the indicated timepoints. E, Levels of select metabolites from glycolysis, glutaminolysis, the TCA cycle, and amino acid metabolism pathways that feed into the TCA cycle; G-6-P, glucose-6-phosphate; DHAP, dihydroxyacetone phosphate; 2/3-PGA, 2/3-phosphoglyceric acid; αKG, alpha-ketoglutarate. Data in A were analyzed by a one-way ANOVA with a Dunnett multiple comparisons; data in B were analyzed by a t test. Metabolomics data were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001 in A and B . *, FDR < 0.05 in E ; n.s., not significant).

Journal: Cancer Immunology Research

Article Title: TCR-Independent Metabolic Reprogramming Precedes Lymphoma-Driven Changes in T-cell Fate

doi: 10.1158/2326-6066.CIR-21-0813

Figure Lengend Snippet: Metabolic reprogramming of CD4 + T cells occurs early during Eμ- Myc lymphoma progression. A, Glu-Cy5 uptake capacity in CD4 + T cells from Eμ- Myc lymphoma-bearing C57BL/6J mice at the indicated timepoints, labeled ex vivo ( n = 5–10 mice). C, Control; E, early; L, late. B, Glu-Cy5 uptake capacity in CD45.2 + CD4 + T cells transferred into normal or Eμ- Myc lymphoma-bearing CD45.1 + congenic mice as in ( n = 5 mice). C, PCA based on untargeted metabolomics of isolated CD4 + T cells from Eμ- Myc lymphoma-bearing C57BL/6J mice at the indicated timepoints ( n = 6–7 mice). D, Heatmap of 59 metabolites that were differentially abundant by ANOVA at the indicated timepoints. E, Levels of select metabolites from glycolysis, glutaminolysis, the TCA cycle, and amino acid metabolism pathways that feed into the TCA cycle; G-6-P, glucose-6-phosphate; DHAP, dihydroxyacetone phosphate; 2/3-PGA, 2/3-phosphoglyceric acid; αKG, alpha-ketoglutarate. Data in A were analyzed by a one-way ANOVA with a Dunnett multiple comparisons; data in B were analyzed by a t test. Metabolomics data were analyzed using PCA and ANOVA in Metaboanalyst. Each dot indicates a biologically independent sample, and all data are mean ± SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001 in A and B . *, FDR < 0.05 in E ; n.s., not significant).

Article Snippet: The Metabolomics Quality Control (QC) Kit, which contains 14 stable isotope-labeled metabolite standards (Cambridge Isotope Labs), included the following compounds: L-alanine ( 13 C3, 99% purity), L-leucine ( 13 C6, 99%), L-phenylalanine ( 13 C6, 99%), L-tryptophan ( 13 C11, 99%), L-tyrosine ( 13 C6, 99%), caffeine ( 13 C3, 99%), D-glucose ( 13 C6, 99%), benzoate ( 13 C6, 99%), citrate ( 13 C3, 99%), octanoate ( 13 C8, 99%), propionate ( 13 C3, 99%), stearic acid ( 13 C18, 98%), succinic acid ( 13 C4, 99%), and D-sucrose ( 13 C6, 98%).

Techniques: Labeling, Ex Vivo, Control, Isolation